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Article

In vitro selection of the Naegleria GIR1 ribozyme identifies three base changes that dramatically improve activity.

RNA (New York, N.Y.) - 1 Dec 1998

Jabri E, Cech T R

Abstract excerpt

NanGIR1 is a member of a new class of group I ribozymes whose putative biological function is site-specific hydrolysis at an internal processing site (IPS). We have previously shown that NanGIR1 requires 1 M KCl for maximal activity, which is nevertheless slow (0.03 min(-1)). We used in vitro selection and an RNA pool with approximately nine mutations per molecule to select for faster hydrolysis at the IPS in 100...

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Identifiers

PMID: 9848647
DOI: 10.1017/s1355838298981237
PMCID: PMC1369719

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