In vitro selection of the Naegleria GIR1 ribozyme identifies three base changes that dramatically improve activity.

doi:10.1017/s1355838298981237

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Article

In vitro selection of the Naegleria GIR1 ribozyme identifies three base changes that dramatically improve activity.

RNA (New York, N.Y.) - 1 Dec 1998

Jabri E, Cech T R

Abstract excerpt

NanGIR1 is a member of a new class of group I ribozymes whose putative biological function is site-specific hydrolysis at an internal processing site (IPS). We have previously shown that NanGIR1 requires 1 M KCl for maximal activity, which is nevertheless slow (0.03 min(-1)). We used in vitro sel...

Topics

Animals
Base Sequence
Catalysis
Electrophoresis, Polyacrylamide Gel
Enzyme Stability
Hot Temperature
Hydrolysis
Molecular Sequence Data
Mutation
Naegleria
Nucleic Acid Conformation
RNA, Catalytic
Sequence Homology, Nucleic Acid

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