Article
Cell lines transfected with the TAP inhibitor ICP47 allow testing peptide binding to a variety of HLA class I molecules.
International immunology - 1 Nov 1998
Gatfield J, Lammert E, Nickolaus P, Münz C, Rothenfusser S, Fisch P, Stevanović S, Schild H, Rammensee H G, Arnold D
Abstract excerpt
The immediate early protein ICP47 of the Herpes simplex virus is known to block the human transporter associated with antigen processing (TAP), thereby creating a TAP-deficient phenotype in any human cell transfected with the corresponding cDNA. Exploiting this inhibitory activity, we constructed...
Topics
- ATP Binding Cassette Transporter, Subfamily B, Member 2
- ATP-Binding Cassette Transporters
- Alleles
- Amino Acid Sequence
- Animals
- Cell Line
- Female
- Flow Cytometry
- HLA-A Antigens
- HLA-B Antigens
- Herpesvirus 2, Human
- Histocompatibility Antigens Class I
- Humans
- Immediate-Early Proteins