Article
A deletion mutant of the type IC restriction endonuclease EcoR1241 expressing a novel DNA specificity.
Nucleic acids research - 25 Sept 1993
Abadjieva A, Patel J, Webb M, Zinkevich V, Firman K
Abstract excerpt
We have developed a complementation assay which allows us to distinguish between mutations affecting subunit assembly and mutations affecting DNA binding in the DNA recognition subunit (HsdS) of the multimeric restriction endonuclease EcoR1241. A number of random point mutations were constructed...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA (Cytosine-5-)-Methyltransferases
- DNA-Binding Proteins
- Deoxyribonucleases, Type I Site-Specific
- Escherichia coli
- Methylation
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Sequence Deletion
- Structure-Activity Relationship
- Substrate Specificity