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Article

A deletion mutant of the type IC restriction endonuclease EcoR1241 expressing a novel DNA specificity.

Nucleic acids research - 25 Sept 1993

Abadjieva A, Patel J, Webb M, Zinkevich V, Firman K

Abstract excerpt

We have developed a complementation assay which allows us to distinguish between mutations affecting subunit assembly and mutations affecting DNA binding in the DNA recognition subunit (HsdS) of the multimeric restriction endonuclease EcoR1241. A number of random point mutations were constructed to test the validity of this assay. Two of the mutants produced were found to be truncated polypeptides that were still...

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Identifiers

PMID: 8233776
DOI: 10.1093/nar/21.19.4435
PMCID: PMC311173

Genes

GAA

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Source receipt: pubmed, retrieved 7 Sept 2026.

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Research trail→

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A deletion mutant of the type IC restriction endonuclease EcoR1241 expressing a novel DNA specificity.PMID 8233776Read ↗

Isolation of a non-classical mutant of the DNA recognition subunit of the type I restriction endonuclease R.EcoR124I.Semantic proximity Read ↗

Characterization of an EcoR124I restriction-modification enzyme produced from a deleted form of the DNA-binding subunit, which results in a novel DNA specificity.Semantic proximity Read ↗

A novel mutant of the type I restriction-modification enzyme EcoR124I is altered at a key stage of the subunit assembly pathway.Semantic proximity Read ↗

The DNA recognition subunit of the type IB restriction-modification enzyme EcoAI tolerates circular permutions of its polypeptide chain.Semantic proximity Read ↗

The effect of recA mutation on the expression of EcoKI and EcoR124I hsd genes cloned in a multicopy plasmid.Semantic proximity Read ↗

Regulation of the activity of the type IC EcoR124I restriction enzyme.Semantic proximity Read ↗

Target recognition by EcoKI: the recognition domain is robust and restriction-deficiency commonly results from the proteolytic control of enzyme activity.Semantic proximity Read ↗

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Structural model for the multisubunit Type IC restriction-modification DNA methyltransferase M.EcoR124I in complex with DNA.Semantic proximity Read ↗

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