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Article

Restriction endonucleases R.EcoKI and R.EcoR124I are probably located in different environments within the bacterial cell.

Folia microbiologica - 1 Jan 1994

Hubácek J, Weiserová M, Janscák P, Firman K

Abstract excerpt

We describe the phenomenon of a transient state of R124I restriction deficiency after long-term storage of the E. coli[pCP1005] strain at 4 degrees C, or after growth of the culture in synthetic M9 medium with the nonmutagenic solvent dimethyl sulfoxide. The unusual high reversion from the R+ 124 to the R- 124 phenotype was observed only in E. coli strain transformed with the high-copy number plasmid pCP1005...

Topics

Cell Membrane
DNA Restriction Enzymes
Deoxyribonucleases, Type I Site-Specific
Dimethyl Sulfoxide
Escherichia coli
Genes, Bacterial
Mutation
Phenotype
Plasmids
Subcellular Fractions
Transformation, Genetic

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Identifiers

PMID: 7959434
DOI: 10.1007/BF02906815

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7959434-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Restriction endonucleases R.EcoKI and R.EcoR124I are probably located in different environments within the bacterial cell.PMID 7959434Read ↗

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