Article
Site-directed incorporation of fluorescent nonnatural amino acids into streptavidin for highly sensitive detection of biotin.
Biomacromolecules - 1 Jan 2000
Murakami H, Hohsaka T, Ashizuka Y, Hashimoto K, Sisido M
Abstract excerpt
Fluorescent nonnatural amino acids were incorporated into specific positions of streptavidin. The positions of the nonnatural amino acids were directed by a CGGG/CCCG four-base codon/anticodon pair. The nonnatural mutants with a single 2-anthrylalanine at the 22nd, 43rd, 54th, and 120th positions, respectively, were found to bind biotin, indicating that the mutants retained active conformation. The fluorescence...
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