Second-site reversion analysis is not a reliable method to determine distances in membrane proteins: an assessment using mutations in yeast cytochrome c oxidase subunits I and II.

doi:10.1006/jmbi.1998.2132

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Article

Second-site reversion analysis is not a reliable method to determine distances in membrane proteins: an assessment using mutations in yeast cytochrome c oxidase subunits I and II.

Journal of molecular biology - 6 Nov 1998

Meunier B, Rich P R

Abstract excerpt

We have examined deficiency mutations and reversions in subunits I and II of yeast cytochrome c oxidase in order to test the reliability of second-site reversion analysis in prediction of tertiary structure of a membrane protein complex. It appears that the method can not provide information on d...

Topics

Animals
Cattle
Electron Transport Complex IV
Fungal Proteins
Membrane Proteins
Models, Molecular
Mutation
Paracoccus
Protein Conformation

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