Alteration of T4 lysozyme structure by second-site reversion of deleterious mutations.

doi:10.1002/pro.5560061115

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Article

Alteration of T4 lysozyme structure by second-site reversion of deleterious mutations.

Protein science : a publication of the Protein Society - 1 Nov 1997

Poteete A R, Rennell D, Bouvier S E, Hardy L W

Abstract excerpt

Mutations that suppress the defects introduced into T4 lysozyme by single amino acid substitutions were isolated and characterized. Among 53 primary sites surveyed, 8 yielded second-site revertants; a total of 18 different mutants were obtained. Most of the restorative mutations exerted global ef...

Topics

Bacteriophage P22
Bacteriophage T4
Genes, Viral
Muramidase
Mutation
Recombinant Proteins
Salmonella typhimurium
Suppression, Genetic

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