Article
Alteration of T4 lysozyme structure by second-site reversion of deleterious mutations.
Protein science : a publication of the Protein Society - 1 Nov 1997
Poteete A R, Rennell D, Bouvier S E, Hardy L W
Abstract excerpt
Mutations that suppress the defects introduced into T4 lysozyme by single amino acid substitutions were isolated and characterized. Among 53 primary sites surveyed, 8 yielded second-site revertants; a total of 18 different mutants were obtained. Most of the restorative mutations exerted global effects, generally increasing lysozyme function in a number of primary mutant contexts. Six of them were more specific,...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.