Constraints on amino acid substitutions in the N-terminal helix of cytochrome c explored by random mutagenesis.

doi:10.1021/bi00099a028

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Article

Constraints on amino acid substitutions in the N-terminal helix of cytochrome c explored by random mutagenesis.

Biochemistry - 3 Sept 1991

Auld D S, Pielak G J

Abstract excerpt

The interaction of the N- and C-terminal helices is a hallmark of the cytochrome c family. Oligodeoxyribonucleotide-directed random mutagenesis within the gene encoding the C102T protein variant of Saccharomyces cerevisiae iso-1-cytochrome c was used to generate a library of mutations at the evol...

Topics

Amino Acid Sequence
Amino Acids
Base Sequence
Cytochrome c Group
Genomic Library
Glycine
Molecular Sequence Data
Mutagenesis
Phenotype
Phenylalanine
Protein Conformation
Saccharomyces cerevisiae

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