Article
Rapid analysis of allelic variants of the sheep PrP gene by oligonucleotide probes.
Microbiology and immunology - 1 Jan 1998
Ishiguro N, Shinagawa M, Onoe S, Yamanouchi K, Saito T
Abstract excerpt
A rapid method to determine the allelic variants of the sheep PrP gene was developed. DNA samples from 128 Suffolk sheep (39 rams and 89 ewes) were screened by using polymerase chain reactions and dot-blot hybridization with 32P-labeled nine allele-specific oligonucleotide probes corresponding to the polymorphic PrP codons 112, 136, 154 and 171. Three allelic variants of the PrP gene, PrP(MARQ), PrP(TARQ) and...
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