Rapid analysis of allelic variants of the sheep PrP gene by oligonucleotide probes.

doi:10.1111/j.1348-0421.1998.tb02327.x

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Article

Rapid analysis of allelic variants of the sheep PrP gene by oligonucleotide probes.

Microbiology and immunology - 1 Jan 1998

Ishiguro N, Shinagawa M, Onoe S, Yamanouchi K, Saito T

Abstract excerpt

A rapid method to determine the allelic variants of the sheep PrP gene was developed. DNA samples from 128 Suffolk sheep (39 rams and 89 ewes) were screened by using polymerase chain reactions and dot-blot hybridization with 32P-labeled nine allele-specific oligonucleotide probes corresponding to...

Topics

Alleles
Animals
DNA Mutational Analysis
Disease Susceptibility
Female
Genetic Variation
Male
Nucleic Acid Hybridization
Oligonucleotide Probes
Polymerase Chain Reaction
Polymorphism, Genetic
PrPSc Proteins
Scrapie
Sheep

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