Article
PrP genotype frequencies of Quebec sheep breeds determined by real-time PCR and molecular beacons.
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire - 1 Jul 2008
L'Homme Yvan, Leboeuf Anne, Cameron Johanne
Abstract excerpt
The allele and genotype frequencies of the prion protein gene (PrP), known to have an impact on scrapie susceptibility, were determined by real-time PCR for 500 Quebec purebred rams. Molecular beacons were very efficient in discriminating the 5 alleles investigated. Polymorphisms at coding positions 136, 154, and 171 of the PrP gene were analyzed using 3 separate real-time PCR reactions and a total of 7 molecular...
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