Article
PrP polymorphisms in Basque sheep breeds determined by PCR-restriction fragment length polymorphism and real-time PCR.
The Veterinary record - 5 Jun 2004
Garcia-Crespo D, Oporto B, Gomez N, Nagore D, Benedicto L, Juste R A, Hurtado A
Abstract excerpt
Two new PCR-based methods were developed to decode prion protein (PrP) gene polymorphisms at codons 136, 154 and 171: a PCR-restriction fragment length polymorphism (RFLP) analysis consisting of two PCR reactions followed by three enzymatic digestions, and a real-time PCR consisting of four reactions with seven fluorogenic probes. Both methods were used to study the distribution of PrP gene polymorphisms in a...
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