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Article

Multiplex real-time PCR method to identify Shiga toxin genes stx1 and stx2 and Escherichia coli O157:H7/H- serotype.

Applied and environmental microbiology - 1 Oct 2003

Jinneman Karen C, Yoshitomi Ken J, Weagant Stephen D

Abstract excerpt

A multiplex real-time PCR method to simultaneously detect the stx1 and stx2 genes of Shiga toxin-producing Escherichia coli and a unique conserved single-nucleotide polymorphism in the E. coli O157:H7/H- uidA gene has been developed. There is more than 98.6% sensitivity and 100% specificity for all three gene targets based on a panel of 138 isolates. The PCR efficiencies were >/= 1.89, and as few as 6...

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Identifiers

PMID: 14532101
DOI: 10.1128/AEM.69.10.6327-6333.2003
PMCID: PMC201207

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