Polymerase chain reaction amplification, cloning and sequencing of variant Escherichia coli Shiga-like toxin type II operons.

doi:10.1006/mpat.1993.1058

Back to search

Article

Polymerase chain reaction amplification, cloning and sequencing of variant Escherichia coli Shiga-like toxin type II operons.

Microbial pathogenesis - 1 Jul 1993

Paton A W, Paton J C, Manning P A

Abstract excerpt

The polymerase chain reaction (PCR) was used to amplify approximately 1.5 kb segments of DNA containing complete Shiga-like toxin type II operons from Escherichia coli serotypes OX3:H21 and O111:H-. These fragments were cloned and DNA sequence analysis identified further variations compared with...

Topics

Bacterial Toxins
Base Sequence
Cloning, Molecular
DNA Primers
Escherichia coli
Genetic Variation
Genome, Bacterial
Molecular Sequence Data
Operon
Polymerase Chain Reaction
Sequence Analysis, DNA
Shiga Toxin 2

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.