Article
Evaluation of a PCR-restriction fragment length polymorphism (PCR-RFLP) assay for molecular epidemiological study of Shiga toxin-producing Escherichia coli.
The Journal of veterinary medical science - 1 Jul 2011
Sugimoto Norihiko, Shima Kensuke, Hinenoya Atsushi, Asakura Masahiro, Matsuhisa Akio, Watanabe Haruo, Yamasaki Shinji
Abstract excerpt
In this study, we have evaluated our recently developed polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay for the molecular subtyping of Shiga toxin-producing Escherichia coli (STEC). A total of 200 STEC strains including O157 (n=100), O26 (n=50), O111 (n=10), and non-O26/O111/O157 (n=40) serogroups isolated during 2005-2006 in Japan, which were identified to be clonally...
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