Article
Development of a multiplex real-time polymerase chain reaction for simultaneous detection of enterohemorrhagic Escherichia coli and enteropathogenic Escherichia coli strains.
Foodborne pathogens and disease - 1 Jul 2010
Pavlovic Melanie, Huber Ingrid, Skala Henrike, Konrad Regina, Schmidt Herbert, Sing Andreas, Busch Ulrich
Abstract excerpt
A multiplex real-time polymerase chain reaction (PCR) was developed for the simultaneous detection of genes encoding intimin (eae) and all variants of Shiga toxins 1 and 2 (stx1 and stx2) in diagnostic samples. The uidA gene encoding a beta-glucuronidase specific for Escherichia coli and Shigella spp. was included in the multiplex PCR assay as an internal amplification control. The multiplex PCR was tested on 30...
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