Article
Collagen binding activity of recombinant and N-terminally modified annexin V (anchorin CII).
Journal of cellular biochemistry - 1 Jun 1995
Turnay J, Pfannmüller E, Lizarbe M A, Bertling W M, von der Mark K
Abstract excerpt
We have cloned the full coding cDNA sequence of chicken annexin V and of a mutant lacking 8 amino acid residues of the N-terminal tail for prokaryotic expression. Both proteins were synthesized in Escherichia coli upon induction with isopropyl thio-beta-D-galactoside, and were purified following two different protocols: one based on the ability of these proteins to interact reversibly with liposomes in the...
Topics
- Animals
- Annexin A5
- Base Sequence
- Chickens
- Cloning, Molecular
- Collagen
- Escherichia coli
- Molecular Sequence Data
- Mutation
- Phospholipids
- Protein Binding
- Recombinant Proteins
