Article
Collagen binding activity of recombinant and N-terminally modified annexin V (anchorin CII).
Journal of cellular biochemistry - 1 Jun 1995
Turnay J, Pfannmüller E, Lizarbe M A, Bertling W M, von der Mark K
Abstract excerpt
We have cloned the full coding cDNA sequence of chicken annexin V and of a mutant lacking 8 amino acid residues of the N-terminal tail for prokaryotic expression. Both proteins were synthesized in Escherichia coli upon induction with isopropyl thio-beta-D-galactoside, and were purified following two different protocols: one based on the ability of these proteins to interact reversibly with liposomes in the...
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