Article
HIV, but not murine leukemia virus, vectors mediate high efficiency gene transfer into freshly isolated G0/G1 human hematopoietic stem cells.
Proceedings of the National Academy of Sciences of the United States of America - 29 Sept 1998
Uchida N, Sutton R E, Friera A M, He D, Reitsma M J, Chang W C, Veres G, Scollay R, Weissman I L
Abstract excerpt
Recent studies have opened the possibility that quiescent, G0/G1 hematopoietic stem cells (HSC) can be gene transduced; lentiviruses (such as HIV type 1, HIV) encode proteins that permit transport of the viral genome into the nucleus of nondividing cells. We and others have recently demonstrated...
Topics
- Antigens, CD34
- Base Sequence
- Colony-Forming Units Assay
- DNA Primers
- G1 Phase
- Gene Expression
- Gene Transfer Techniques
- Genes, Reporter
- Genetic Therapy
- Genetic Vectors
- Green Fluorescent Proteins
- HIV-1
- Hematopoietic Stem Cells