Article
The late dividing population of gamma-retroviral vector transduced human mobilized peripheral blood progenitor cells contributes most to gene-marked cell engraftment in nonobese diabetic/severe combined immunodeficient mice.
Stem cells (Dayton, Ohio) - 1 Jul 2007
Brenner Sebastian, Ryser Martin F, Whiting-Theobald Narda L, Gentsch Marcus, Linton Gilda F, Malech Harry L
Abstract excerpt
We used the nonobese diabetic/severe combined immunodeficient (NOD/SCID) mouse model to assess the repopulation potential of subpopulations of mobilized human CD34+ peripheral blood progenitor cells (PBPC). First, PBPC were transduced with gamma-retrovirus vector RD114-MFGS-CFP, which requires cell division for successful transduction, at 24 hours, 48 hours, and 72 hours to achieve 96% cyan fluorescent protein...
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