Application of automated methods for determination of protein conformational stability.

doi:10.1016/s0076-6879(98)95039-x

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Article

Application of automated methods for determination of protein conformational stability.

Methods in enzymology - 1 Jan 1998

Schwehm J M, Stites W E

Abstract excerpt

Automation of protein stability determination saves much time and often results in better data. In our laboratory we estimate that the lack of such instrumentation would increase the amount of time required to make and characterize a mutant protein by at least one-third. As with any experiment, l...

Topics

Enzyme Stability
Fluorometry
Guanidine
Hot Temperature
Hydrogen-Ion Concentration
Micrococcal Nuclease
Mutation
Protein Conformation
Protein Denaturation
Reproducibility of Results
Solvents
Titrimetry

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