Article
Altered DNA-binding specificity mutants of EKLF and Sp1 show that EKLF is an activator of the beta-globin locus control region in vivo.
Genes & development - 15 Sept 1998
Gillemans N, Tewari R, Lindeboom F, Rottier R, de Wit T, Wijgerde M, Grosveld F, Philipsen S
Abstract excerpt
The locus control region of the beta-globin cluster contains five DNase I hypersensitive sites (5'HS1-5) required for locus activation. 5'HS3 contains six G-rich motifs that are essential for its activity. Members of a protein family, characterized by three zinc fingers highly homologous to those...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Binding Sites
- CHO Cells
- Cell Line
- Cricetinae
- DNA
- DNA-Binding Proteins
- Deoxyribonuclease I
- Erythrocytes
- Gene Expression
- Genes, Reporter
- Globins
- Humans
- In Situ Hybridization