Article
Clonal analysis of a human antibody response. III. Nucleotide sequences of monoclonal IgM, IgG, and IgA to rabies virus reveal restricted V kappa gene utilization, junctional V kappa J kappa and V lambda J lambda diversity, and somatic hypermutation.
Journal of immunology (Baltimore, Md. : 1950) - 15 Sept 1998
Ikematsu W, Kobarg J, Ikematsu H, Ichiyoshi Y, Casali P
Abstract excerpt
In previous work, we generated four IgM, five IgG1, and one IgA1 mAbs to rabies virus using B cells from four subjects vaccinated with inactivated rabies virus, a thymus-dependent (TD) mosaic Ag, and sequenced the mAb V(H)DJ(H) genes. Here, we have cloned the V kappa J kappa and V lambda J lambda...
Topics
- Amino Acid Sequence
- Antibodies, Monoclonal
- Antibodies, Viral
- Antibody Diversity
- Base Sequence
- Clone Cells
- Cloning, Molecular
- Gene Rearrangement, B-Lymphocyte, Light Chain
- Genes, Immunoglobulin
- HIV Antibodies
- Haemophilus influenzae type b
- Humans
- Immunoglobulin A