V-gene amplification revisited - An optimised procedure for amplification of rearranged human antibody genes of different isotypes.

doi:10.1016/j.nbt.2010.01.001

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Article

V-gene amplification revisited - An optimised procedure for amplification of rearranged human antibody genes of different isotypes.

New biotechnology - 31 May 2010

Lim Theam Soon, Mollova Svetlana, Rubelt Florian, Sievert Volker, Dübel Stefan, Lehrach Hans, Konthur Zoltán

Abstract excerpt

For studying human antibody variable (V)-gene usage in any group of individuals or for the generation of recombinant human antibody libraries for phage display, quality and yield of the amplified V-gene repertoire is of utmost importance. Key parameters affecting the amplification of full antibody repertoires are V-gene specific primer design, complementary DNA (cDNA) synthesis from total RNA extracts of...

Topics

Antibodies
Base Sequence
DNA Primers
Genetic Variation
Humans
Immunoglobulin Variable Region
Molecular Sequence Data
Polymerase Chain Reaction
Sequence Analysis, DNA
Single-Chain Antibodies

Source

Open in PubMed

Identifiers

PMID: 20083243
DOI: 10.1016/j.nbt.2010.01.001

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

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