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V-gene amplification revisited - An optimised procedure for amplification of rearranged human antibody genes of different isotypes.

New biotechnology - 31 May 2010

Lim Theam Soon, Mollova Svetlana, Rubelt Florian, Sievert Volker, Dübel Stefan, Lehrach Hans, Konthur Zoltán

Abstract excerpt

For studying human antibody variable (V)-gene usage in any group of individuals or for the generation of recombinant human antibody libraries for phage display, quality and yield of the amplified V-gene repertoire is of utmost importance. Key parameters affecting the amplification of full antibody repertoires are V-gene specific primer design, complementary DNA (cDNA) synthesis from total RNA extracts of...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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PMID: 20083243
DOI: 10.1016/j.nbt.2010.01.001

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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