Article
A TRP1-marker-based system for gene complementation, overexpression, reporter gene expression and gene modification in Candida glabrata.
FEMS yeast research - 6 Jan 2021
Sprenger Marcel, Brunke Sascha, Hube Bernhard, Kasper Lydia
Abstract excerpt
Although less prevalent than its relative Candida albicans, the yeast Candida glabrata is a successful pathogen of humans, which causes life-threatening candidiasis. It is thus vital to understand the pathogenicity mechanisms and contributing genes in C. glabrata. However, gene complementation as a tool for restoring the function of a previously deleted gene is not standardized in C. glabrata, and it is less...
Topics
- Candida glabrata
- Genes, Reporter
- Genetic Complementation Test
- Genetic Engineering
- Mutation
- Tryptophan
