Article
Identification of genetic loci implicated in the survival of Mycobacterium smegmatis in human mononuclear phagocytes.
Molecular microbiology - 1 Jul 1998
Lagier B, Pelicic V, Lecossier D, Prod'hom G, Rauzier J, Guilhot C, Gicquel B, Hance A J
Abstract excerpt
A luminescence-based procedure that permits the rapid evaluation of the survival of mycobacteria within mononuclear phagocytes was developed and used to screen insertional mutants of Mycobacterium smegmatis for their ability to survive in human monocyte-derived macrophages. Among the 5000 mutants...
Topics
- Base Sequence
- DNA Transposable Elements
- Genes, Bacterial
- Genetic Complementation Test
- Humans
- Luminescent Measurements
- Macrophages
- Microbiological Techniques
- Molecular Sequence Data
- Mutation
- Mycobacterium smegmatis
- Mycobacterium tuberculosis
- Phagocytes