Article
Acquisition of the ts phenotype by a chemically mutagenized cold-passaged human respiratory syncytial virus vaccine candidate results from the acquisition of a single mutation in the polymerase (L) gene.
Virus genes - 1 Jan 1996
Crowe J E, Firestone C Y, Whitehead S S, Collins P L, Murphy B R
Abstract excerpt
A cold-passaged (cp) temperature-sensitive (ts) mutant of human respiratory syncytial virus designated RSV cpts-248 was previously derived by random chemical mutagenesis of the non-ts mutant cp-RSV that possesses one or more host range mutations. We previously demonstrated in rodents and seronega...
Topics
- Animals
- Base Sequence
- Chlorocebus aethiops
- Cold Temperature
- DNA, Viral
- DNA-Directed RNA Polymerases
- Genes, Viral
- Humans
- Molecular Sequence Data
- Mutagenesis
- Phenotype
- Respiratory Syncytial Virus, Human
- Vero Cells
- Viral Proteins
- Viral Vaccines