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Article

Human N-demethylation of (S)-mephenytoin by cytochrome P450s 2C9 and 2B6.

Drug metabolism and disposition: the biological fate of chemicals - 1 Aug 1998

Ko J W, Desta Z, Flockhart D A

Abstract excerpt

We tested the ability of human liver microsomes (HLMs) and recombinant human cytochrome P450 (CYP or P450) isoforms to catalyze the N-demethylation of nirvanol-free (S)-mephenytoin [(S)-MP] in vitro. In mixed HLMs, the kinetics of (S)-MP N-demethylation suggested two contributing activities. A high-affinity/low-capacity component exhibited a KM of 174.1 microM and a Vmax of 170.5 pmol/mg protein/min, whereas a...

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PMID: 9698292

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