Article
Lack of low Km diazepam N-demethylase in livers of poor metabolizers for S-mephenytoin 4'-hydroxylation.
Pharmacogenetics - 1 Dec 1994
Yasumori T, Li Q H, Yamazoe Y, Ueda M, Tsuzuki T, Kato R
Abstract excerpt
Metabolism of diazepam was studied in vitro to identify the forms of cytochrome P450 (CYP) responsible for N-demethylation (nordazepam formation) and 3-hydroxylation (temazepam formation), using liver microsomes obtained from extensive (EM) and poor metabolizers (PM) for S-mephenytoin 4'-hydroxylation. Involvement of at least two P450 forms in diazepam N-demethylation was suggested by a biphasic pattern in...
Topics
- Antibodies
- Aryl Hydrocarbon Hydroxylases
- Cytochrome P-450 CYP2C19
- Cytochrome P-450 CYP2E1
- Cytochrome P-450 Enzyme System
- Diazepam
- Humans
- Hydroxylation
- In Vitro Techniques
- Kinetics
- Mephenytoin