Article
DAMGO recognizes four residues in the third extracellular loop to discriminate between mu- and kappa-opioid receptors.
European journal of pharmacology - 5 Jun 1998
Seki T, Minami M, Nakagawa T, Ienaga Y, Morisada A, Satoh M
Abstract excerpt
Previously, we reported that replacement of the region from the fifth transmembrane domain to the C-terminus of kappa-opioid receptor with the corresponding region of mu-opioid receptor gives high affinity for [D-Ala2, N-MePhe4, Gly-ol5]enkephalin (DAMGO), a mu-opioid receptor-selective ligand, t...
Topics
- Adenylyl Cyclases
- Amino Acid Sequence
- Animals
- Enkephalin, Ala(2)-MePhe(4)-Gly(5)-
- Enkephalins
- Extracellular Space
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Protein Structure, Secondary
- Radioligand Assay
- Rats
- Receptors, Opioid, kappa
- Receptors, Opioid, mu
- Recombinant Fusion Proteins