Article
Overexpression and lack of degradation of thaumatin in an aspergillopepsin A-defective mutant of Aspergillus awamori containing an insertion in the pepA gene.
Applied microbiology and biotechnology - 1 Dec 2000
Moralejo F J, Cardoza R E, Gutiérrez S, Sisniega H, Faus I, Martín J F
Abstract excerpt
A gene encoding the sweet-tasting protein thaumatin (tha) with optimized codon usage was expressed in Aspergillus awamori. Mutants of A. awamori with reduced proteolytic activity were isolated. One of these mutants, named lpr66, contained an insertion of about 200 bp in the pepA gene, resulting in an inactive aspergillopepsin A. In vitro thaumatin degradation tests confirmed that culture broths of mutant lpr66...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.