Article
Analysis of p53 gene mutation by polymerase chain reaction-single strand conformation polymorphism provides independent prognostic information in node-negative breast cancer.
Clinical cancer research : an official journal of the American Association for Cancer Research - 1 Jul 1998
Iacopetta B, Grieu F, Powell B, Soong R, McCaul K, Seshadri R
Abstract excerpt
Controversy continues regarding the prognostic utility of detection of p53 gene abnormalities in node-negative breast cancer. To resolve this, we used a rapid and nonisotopic PCR-single strand conformation polymorphism method to screen for mutations in exons 4-8 of the p53 gene in primary tumors from 422 node-negative breast cancer patients. The prevalence of p53 mutation in the exons tested was 18%. p53 mutation...
Topics
- Adult
- Aged
- Aged, 80 and over
- Breast Neoplasms
- Exons
- Female
- Gene Amplification
- Genes, p53
- Humans
- Middle Aged
- Mutation
- Polymerase Chain Reaction
- Polymorphism, Single-Stranded Conformational
- Prognosis