Article
Competitive oligonucleotide single-base extension combined with mass spectrometric detection for mutation screening.
BioTechniques - 1 Oct 1997
Higgins G S, Little D P, Köster H
Abstract excerpt
A rapid, robust and widely applicable mutation detection scheme not requiring radioactivity or gel-based detection is introduced. It is a single-tube, competitive oligonucleotide single-base extension (COSBE) reaction using a pair of primers with the 3'-terminal base complementary to either the normal or mutant allele. Upon hybridization and addition of a polymerase and the nucleotide triphosphate corresponding...
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