Article
Competitive oligonucleotide single-base extension combined with mass spectrometric detection for mutation screening.
BioTechniques - 1 Oct 1997
Higgins G S, Little D P, Köster H
Abstract excerpt
A rapid, robust and widely applicable mutation detection scheme not requiring radioactivity or gel-based detection is introduced. It is a single-tube, competitive oligonucleotide single-base extension (COSBE) reaction using a pair of primers with the 3'-terminal base complementary to either the n...
Topics
- Alleles
- Base Composition
- Binding, Competitive
- Cystic Fibrosis Transmembrane Conductance Regulator
- DNA Mutational Analysis
- DNA Primers
- Humans
- Mass Spectrometry
- Nucleic Acid Hybridization
- Oligonucleotides
- Polymerase Chain Reaction
- Reproducibility of Results
- Sensitivity and Specificity