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Article

Mutation screening by a combination of biotin-SSCP and direct sequencing.

Human genetics - 1 Mar 1994

Virdi A S, Loughlin J A, Irven C M, Goodship J, Sykes B C

Abstract excerpt

We have developed a mutation detection strategy that combines single strand conformational polymorphism (SSCP) analysis of one strand of a double-stranded amplification product with direct sequencing of the other. Using this strategy, which we find economical of both time and resources, we have identified a G to A transition, which substitutes a serine for glycine residue at position 862 in the major helix of the...

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PMID: 8125479
DOI: 10.1007/BF00212024

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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