Biochemical characterization and localization of transglutaminase in wild-type and cell-death mutants of the nematode Caenorhabditis elegans.

doi:10.1046/j.1432-1327.1998.2530583.x

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Article

Biochemical characterization and localization of transglutaminase in wild-type and cell-death mutants of the nematode Caenorhabditis elegans.

European journal of biochemistry - 1 May 1998

Mádi A, Punyiczki M, di Rao M, Piacentini M, Fésüs L

Abstract excerpt

Transglutaminase activity was characterized in extracts of the nematode Caenorhabditis elegans using a microtiter plate method, and found to be Ca2+-dependent, optimal at pH 8.0, and to be inhibited by EGTA, ammonia, iodoacetamide and GTP. Monoclonal and polyclonal antibodies raised against human...

Topics

Animals
Antibodies
Antibodies, Monoclonal
Caenorhabditis elegans
Cell Death
Humans
Immunohistochemistry
Kinetics
Larva
Molecular Weight
Mutation
Ovum
Transglutaminases

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