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Article

Whole-virus vaccine development by continuous culture on a complementing host.

Bio/technology (Nature Publishing Company) - 1 Jun 1995

Kong D, Yin J

Abstract excerpt

We have evaluated an adaptive strategy for generating whole-virus vaccines using a bacteriophage model. Wildtype phage T7 was cultivated in a two-stage continuous stirred-tank reactor (CSTR) utilizing a recombinant E. coli host that constitutively expressed T7 RNA polymerase, an essential enzyme of the early viral metabolism. Over the course of 180 generations a diversity of phage variants emerged, outgrew the...

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PMID: 9634797
DOI: 10.1038/nbt0695-583

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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