Article
Interactions between novel tumor necrosis factor-alpha mutants and receptors on tumor and normal cells.
Agricultural and biological chemistry - 1 Jan 1991
Nakamura S, Masegi T, Fukuoka M, Kitai K, Kato A, Ichikawa Y, Watanabe N, Niitsu Y
Abstract excerpt
We prepared several TNF mutants by protein engineering techniques and compared their biological properties with those of the wild-type TNF. The mutant that lacked 7 N-terminal amino acids had higher cytotoxicity and higher binding activity to receptors on tumor cells. In contrast, the mutagenesis of Arg32 or Ala84, in combination with the deletion of 7 N-terminal amino acids, eliminated the cytotoxicity and the...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.