Article
Biological activities of human tumor necrosis factor-alpha and its novel mutants.
Biochemistry and molecular biology international - 1 May 1996
Xi T, Shi X, Guo D, Dong X, Xu X, Zhu D
Abstract excerpt
Biological activities of human tumor necrosis factor-alpha (hTNF-alpha) and its mutants were compared. In cytotoxicity assay with L929 cells, one mutant, designated as TNF-B, showed 4.5-fold higher activity than TNF examined. In receptor binding assay, TNF-B had almost the same affinity for TNF r...
Topics
- Amino Acid Sequence
- Animals
- Binding, Competitive
- DNA, Neoplasm
- Drug Screening Assays, Antitumor
- Fibroblasts
- Humans
- Laryngeal Neoplasms
- Leukemia, Monocytic, Acute
- Mice
- Molecular Sequence Data
- Mutation
- Neoplasm Proteins
- Radioligand Assay
- Receptors, Tumor Necrosis Factor
- Sequence Homology, Amino Acid
- Tumor Cells, Cultured
- Tumor Necrosis Factor-alpha