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Article

High-throughput screening for known mutations by automated analysis of single sequencing reactions.

BioTechniques - 1 May 1998

Kristensen T, Kristensen V N, Børresen-Dale A L

Abstract excerpt

This work shows that single sequencing reactions analyzed on an automated DNA sequencer can be an efficient way of screening PCR products for known mutations. We have analyzed a mutation in exon 10 of the human aromatase gene and show that an unambiguous genotype could be elucidated in more than 90% of the analyzed samples. Compared to analysis by full sequencing, 4 times more samples can be analyzed per gel, so...

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Identifiers

PMID: 9591134
DOI: 10.2144/98245dt04

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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