Article
Genomic replacement in Escherichia coli K-12 using covalently closed circular plasmid DNA.
Gene - 30 Nov 1990
Oden K L, DeVeaux L C, Vibat C R, Cronan J E, Gennis R B
Abstract excerpt
A number of gene replacements at different loci were constructed using covalently closed circular (ccc) plasmid DNA in the recB21 recC22 sbcB15 sbcC201 mutant of Escherichia coli (JC7623). Selected constructs representing deletions and insertion mutations formed from double-crossover events involving the ccc plasmid molecules and the genome were confirmed by Southern blots, and the frequency of double-crossover...
Topics
- Blotting, Southern
- Crossing Over, Genetic
- DNA, Bacterial
- DNA, Circular
- Escherichia coli
- Mutation
- Plasmids