Article
Frequent detection of ras and p53 mutations in brush cytology samples from lung cancer patients by a restriction fragment length polymorphism-based "enriched PCR" technique.
Clinical cancer research : an official journal of the American Association for Cancer Research - 1 Feb 1998
Behn M, Qun S, Pankow W, Havemann K, Schuermann M
Abstract excerpt
RFLP-mediated PCR has been successfully applied as a reliable tool in the detection of ras mutations in many cancers and provides a basis for "mutant-enriched PCR" protocols. We have, therefore, modified this technique to the sensitive detection of K-ras codon 12 and also p53 "hot spot" mutations...
Topics
- Aged
- Aged, 80 and over
- Alleles
- Biopsy
- Codon
- Cytological Techniques
- DNA Primers
- Exons
- Female
- Genes, p53
- Genes, ras
- Humans
- Lung Neoplasms
- Male
- Middle Aged
- Mutation
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length