Article
The role of single-stranded DNA in Flp-mediated strand exchange.
The Journal of biological chemistry - 27 Feb 1998
Zhu X D, Sadowski P D
Abstract excerpt
The Flp recognition target site contains two inverted 13-base pair (bp) Flp binding sequences that surround an 8-bp core region. Flp recombinase has been shown to carry out strand ligation independently of its ability to execute strand cleavage. Using a synthetic activated DNA substrate bearing a 3'-phosphotyrosine group, we have developed an assay to measure strand exchange by Flp proteins. We have shown that...
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