Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Bending-incompetent variants of Flp recombinase mediate strand transfer in half-site recombinations: role of DNA bending in recombination.

Gene - 21 Sept 1992

Chen J W, Evans B, Rosenfeldt H, Jayaram M

Abstract excerpt

One key feature of the interaction of Flp recombinase with its target site (FRT) is the large bend introduced in the substrate as a result of protein binding. The extent of bending was found to depend on the phasing and spacing of the Flp monomers occupying the two Flp-binding elements (FBE) bordering the strand-exchange region (spacer) of the substrate. The relative mobilities of the Flp complexes formed by the...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Base Sequence
Share to Catalysis
Share to DNA, Fungal

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Genetic Variation

Share to Molecular Sequence Data

Share to Nucleic Acid Conformation

Share to Recombination, Genetic

Share to Saccharomyces cerevisiae

Source

Identifiers

PMID: 1398089
DOI: 10.1016/0378-1119(92)90064-v

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Bending-incompetent variants of Flp recombinase mediate strand transfer in half-site recombinations: role of DNA bending in recombination.PMID 1398089Read ↗

DNA sequence determinant for FIp-induced DNA bending.Semantic proximity Read ↗

The role of single-stranded DNA in Flp-mediated strand exchange.Semantic proximity Read ↗

Tyr60 variants of Flp recombinase generate conformationally altered protein-DNA complexes. Differential activity in full-site and half-site recombinations.Semantic proximity Read ↗

Mechanism of site-specific recombination. Logic of assembling recombinase catalytic site from fractional active sites.Semantic proximity Read ↗

Recombination of hybrid target sites by binary combinations of Flp variants: mutations that foster interprotomer collaboration and enlarge substrate tolerance.Semantic proximity Read ↗

Electrostatic suppression allows tyrosine site-specific recombination in the absence of a conserved catalytic arginine.Semantic proximity Read ↗

Non-equivalent interactions between amino-terminal domains of neighboring lambda integrase protomers direct Holliday junction resolution.Semantic proximity Read ↗

Unveiling hidden catalytic contributions of the conserved His/Trp-III in tyrosine recombinases: assembly of a novel active site in Flp recombinase harboring alanine at this position.Semantic proximity Read ↗

Functional analysis of Arg-308 mutants of Flp recombinase. Possible role of Arg-308 in coupling substrate binding to catalysis.Semantic proximity Read ↗

Alternative geometries of DNA looping: an analysis using the SfiI endonuclease.Semantic proximity Read ↗

Conformational changes and cleavage by the homing endonuclease I-PpoI: a critical role for a leucine residue in the active site.Semantic proximity Read ↗

Trans cooperativity by a split DNA recombinase: the central and catalytic domains of bacteriophage lambda integrase cooperate in cleaving DNA substrates when the two domains are not covalently linked.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.