Bending-incompetent variants of Flp recombinase mediate strand transfer in half-site recombinations: role of DNA bending in recombination.

doi:10.1016/0378-1119(92)90064-v

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Article

Bending-incompetent variants of Flp recombinase mediate strand transfer in half-site recombinations: role of DNA bending in recombination.

Gene - 21 Sept 1992

Chen J W, Evans B, Rosenfeldt H, Jayaram M

Abstract excerpt

One key feature of the interaction of Flp recombinase with its target site (FRT) is the large bend introduced in the substrate as a result of protein binding. The extent of bending was found to depend on the phasing and spacing of the Flp monomers occupying the two Flp-binding elements (FBE) bord...

Topics

Base Sequence
Catalysis
DNA Nucleotidyltransferases
DNA, Fungal
Genetic Variation
Molecular Sequence Data
Nucleic Acid Conformation
Recombination, Genetic
Saccharomyces cerevisiae
Tyrosine

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