Article
Mechanism of cleavage and ligation by FLP recombinase: classification of mutations in FLP protein by in vitro complementation analysis.
Molecular and cellular biology - 1 Jun 1993
Pan G, Luetke K, Sadowski P D
Abstract excerpt
The FLP recombinase of the 2 microns plasmid of Saccharomyces cerevisiae is a member of the integrase family of site-specific recombinases. Recombination catalyzed by members of this family proceeds via the ordered cleavage and religation of four strands of DNA. Although the amino acid sequences...
Topics
- Amino Acid Sequence
- Base Sequence
- Binding Sites
- DNA Nucleotidyltransferases
- Fungal Proteins
- Genetic Complementation Test
- Macromolecular Substances
- Models, Genetic
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides
- Saccharomyces cerevisiae
- Substrate Specificity