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Article

Mechanism of cleavage and ligation by FLP recombinase: classification of mutations in FLP protein by in vitro complementation analysis.

Molecular and cellular biology - 1 Jun 1993

Pan G, Luetke K, Sadowski P D

Abstract excerpt

The FLP recombinase of the 2 microns plasmid of Saccharomyces cerevisiae is a member of the integrase family of site-specific recombinases. Recombination catalyzed by members of this family proceeds via the ordered cleavage and religation of four strands of DNA. Although the amino acid sequences of integrase family members are quite different, each recombinase maintains an absolutely conserved tetrad of amino...

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Identifiers

PMID: 8497247
DOI: 10.1128/mcb.13.6.3167-3175.1993
PMCID: PMC359755

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