Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Mechanism of site-specific recombination. Logic of assembling recombinase catalytic site from fractional active sites.

The Journal of biological chemistry - 15 Aug 1993

Lee J, Jayaram M

Abstract excerpt

The active nucleophilic species in the strand cleavage and strand exchange steps of site-specific recombination by the Flp protein are the active site tyrosine (Tyr-343) of Flp and the 5'-hydroxyl of Flp-nicked DNA, respectively. The target phosphodiester, activated by Flp, can be cleaved by an exogenous nucleophile derived, for example, from H2O2. Flp variants that are defective in the phosphate activation step...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Base Sequence
Share to Binding Sites
Share to DNA

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Fungal Proteins

Share to Molecular Sequence Data

Share to Mutation

Share to Recombination, Genetic

Share to Saccharomyces cerevisiae

Source

Identifiers

PMID: 8349636

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Mechanism of site-specific recombination. Logic of assembling recombinase catalytic site from fractional active sites.PMID 8349636Read ↗

Electrostatic suppression allows tyrosine site-specific recombination in the absence of a conserved catalytic arginine.Semantic proximity Read ↗

Unveiling hidden catalytic contributions of the conserved His/Trp-III in tyrosine recombinases: assembly of a novel active site in Flp recombinase harboring alanine at this position.Semantic proximity Read ↗

Mechanism of cleavage and ligation by FLP recombinase: classification of mutations in FLP protein by in vitro complementation analysis.Semantic proximity Read ↗

Generality of the shared active site among yeast family site-specific recombinases. The R site-specific recombinase follows the Flp paradigm [corrected].Semantic proximity Read ↗

Reactions of Cre with methylphosphonate DNA: similarities and contrasts with Flp and vaccinia topoisomerase.Semantic proximity Read ↗

Stereospecific suppression of active site mutants by methylphosphonate substituted substrates reveals the stereochemical course of site-specific DNA recombination.Semantic proximity Read ↗

Restoration of catalytic functions in Cre recombinase mutants by electrostatic compensation between active site and DNA substrate.Semantic proximity Read ↗

The role of single-stranded DNA in Flp-mediated strand exchange.Semantic proximity Read ↗

Conserved Themes but Novel Activities in Recombinases and TopoisomerasesSemantic proximity Read ↗

Attenuating functions of the C terminus of lambda integrase.Semantic proximity Read ↗

Mechanism of homologous recombination: mediators and helicases take on regulatory functionsSemantic proximity Read ↗

The tyrosine-6 hydroxyl of gamma delta resolvase is not required for the DNA cleavage and rejoining reactions.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.