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Article

Mechanism of site-specific recombination. Logic of assembling recombinase catalytic site from fractional active sites.

The Journal of biological chemistry - 15 Aug 1993

Lee J, Jayaram M

Abstract excerpt

The active nucleophilic species in the strand cleavage and strand exchange steps of site-specific recombination by the Flp protein are the active site tyrosine (Tyr-343) of Flp and the 5'-hydroxyl of Flp-nicked DNA, respectively. The target phosphodiester, activated by Flp, can be cleaved by an e...

Topics

Base Sequence
Binding Sites
Catalysis
DNA
DNA Nucleotidyltransferases
Fungal Proteins
Molecular Sequence Data
Mutation
Recombination, Genetic
Saccharomyces cerevisiae
Tyrosine

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PMID: 8349636

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8349636-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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