Article
Tyr60 variants of Flp recombinase generate conformationally altered protein-DNA complexes. Differential activity in full-site and half-site recombinations.
Journal of molecular biology - 5 Mar 1991
Chen J W, Evans B R, Zheng L, Jayaram M
Abstract excerpt
The tyrosine at position 60 of the Flp recombinase of the Saccharomyces cerevisiae plasmid, 2 mu circle, is invariant among site-specific recombinases of the "yeast plasmid family". Alterations of this residue give rise to Flp variants that show no recombination activity when assayed in vivo in E...
Topics
- Base Sequence
- DNA Nucleotidyltransferases
- DNA, Bacterial
- Escherichia coli
- Genetic Variation
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Oligonucleotide Probes
- Plasmids
- Protein Binding
- Recombination, Genetic
- Saccharomyces cerevisiae
- Tyrosine