Article
Abnormal proteins enhance stress-induced cell death.
Biochemical and biophysical research communications - 4 Feb 1998
Kim S J, Kim S
Abstract excerpt
The effect of abnormal proteins on cell viability was studied using artificially cleaved polypeptides. Escherichia coli methionyl-tRNA synthetase (MetRS) consists of two distinct domains and its activity is essential for cell viability. The polypeptide chain was split by linker insertion and expressed as two fragments. Two pairs of polypeptides, one split within the N-terminal domain and another at the junction...
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