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Article

Binding of double-stranded DNA by Escherichia coli RecA protein monitored by a fluorescent dye displacement assay.

Nucleic acids research - 15 Jan 1998

Zaitsev E N, Kowalczykowski S C

Abstract excerpt

We have developed a new assay to characterize the double-stranded DNA (dsDNA) binding properties of RecA protein. This assay is based on measurement of changes in the fluorescence of a 4',6-diamidino-2-phenylindole (DAPI)-dsDNA complex upon RecA protein binding. The binding of RecA protein to a complex of DAPI and dsDNA results in displacement of the bound DAPI, producing a decrease in the observed fluorescence....

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Identifiers

PMID: 9421529
DOI: 10.1093/nar/26.2.650
PMCID: PMC147265

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