Article
Localization of RecA in Escherichia coli K-12 using RecA-GFP.
Molecular microbiology - 1 Aug 2005
Renzette Nicholas, Gumlaw Nathan, Nordman Jared T, Krieger Marlee, Yeh Su-Ping, Long Edward, Centore Richard, Boonsombat Ruethairat, Sandler Steven J
Abstract excerpt
RecA is important in recombination, DNA repair and repair of replication forks. It functions through the production of a protein-DNA filament. To study the localization of RecA in live Escherichia coli cells, the RecA protein was fused to the green fluorescence protein (GFP). Strains with this gene have recombination/DNA repair activities three- to tenfold below wild type (or about 1000-fold above that of a recA...
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