Article
Use of a two-hybrid system to identify mutations in Max that confer increased affinity for Myc.
DNA and cell biology - 1 Nov 1997
Wang H, Van Den Bergh F, Spencer E, Wilcox K, Herman T
Abstract excerpt
A yeast two-hybrid system was used to identify mutants of Max that exhibit an increased affinity for Myc. Truncated forms of the Max helix-loop-helix/leucine zipper motif (HLH/Zip) were first expressed in a two- hybrid system in which the bait protein was the HLH/Zip motif of Myc. Deletion of ami...
Topics
- Bacterial Proteins
- Basic-Leucine Zipper Transcription Factors
- Codon
- DNA-Binding Proteins
- Dimerization
- Genes, myc
- Genetic Vectors
- Helix-Loop-Helix Motifs
- Lac Operon
- Leucine Zippers
- Models, Genetic
- Mutation
- Recombinant Fusion Proteins
- Repressor Proteins
- Saccharomyces cerevisiae
- Serine Endopeptidases
- Transcription Factors
- Transcriptional Activation