Article
How Escherichia coli can bias the results of molecular cloning: preferential selection of defective genomes of hepatitis C virus during the cloning procedure.
Proceedings of the National Academy of Sciences of the United States of America - 9 Dec 1997
Forns X, Bukh J, Purcell R H, Emerson S U
Abstract excerpt
Cloned PCR products containing hepatitis C virus (HCV) genomic fragments have been used for analyses of HCV genomic heterogeneity and protein expression. These studies assume that the clones derived are representative of the entire virus population and that subsets are not inadvertently selected. The aim of the present study was to express HCV structural proteins. However, we found that there was a strong cloning...
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