Article
Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae.
Genetics - 1 Oct 1997
Lussier M, White A M, Sheraton J, di Paolo T, Treadwell J, Southard S B, Horenstein C I, Chen-Weiner J, Ram A F, Kapteyn J C, Roemer T W, Vo D H, Bondoc D C, Hall J, Zhong W W, Sdicu A M, Davies J, Klis F M, Robbins P W, Bussey H
Abstract excerpt
The sequenced yeast genome offers a unique resource for the analysis of eukaryotic cell function and enables genome-wide screens for genes involved in cellular processes. We have identified genes involved in cell surface assembly by screening transposon-mutagenized cells for altered sensitivity to calcofluor white, followed by supplementary screens to further characterize mutant phenotypes. The mutated genes were...
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